B1.1a (Light microscopes)

• Light Microscopes

  A) eyepiece lense

  B) objective lenses

  C) arm

  D) coarse focus wheel

  E) fine focus wheel

  F) lamp

  G) stage + stage clips

  
• Slides + coverslips


  A coverslip is placed over a specimen on a microscope slide, to hold the specimen in place and protect it from contamination from the environment. At the same time, the coverslip protects the lens of the microscope, stopping it from coming into contact with the specimen.
• Staining Specimens


  Specimens are stained to enhance visualization of the cell or to highlight different structures/tissues.
• Total Magnification Equation

  Total magnification = magnification of eyepiece lens x magnification of objective lens
• Magnification of Image Equation


  Magnification of image = size of image / size of object
• Micro meters -> millimeters

  1 μm = 0.001 mm

  1 mm = 1000 μm