In his work, Siffre was the only participant. Siffre noted that when he went into the cave at 60 years old, his body responded very differently, with his body following more of a 48 hour cycle. In contrast, Czeisler has found evidence that cycles vary by as much as 13-65 hours. This means the results may lack generalisability to other individuals and populations since individual differences may affect our rhythms and these haven’t been accounted for