Hemocytometer

• Hemocytometer can either be MANUAL or AUTOMATED
• Hemocytometer is used to perform what on what?

  manual cell counts on body fluids
• What are the body fluids that can utilize the hemocytometer?

  CSF, synovial, pleural, pericardial, peritoneal, peritoneal dialysates, bronchoalveolar lavages, and semen
• What type of body fluid is HIGHLY VISCOUS?

  Synovial fluid
• What type of body fluid can FAIL to LIQUEFY?

  Semen
• Body fluids like Synovial fluid and Semen undergo what before counting?

  pre-treatment
• If the medical technologist decided to continue analysis a CLOTTED sample, what should be noted upon releasing of the result?

  "Specimen clotted; results may be inaccurate and must be interpreted with caution."
• What are the 3 main types of hemocytometer?

  Fusch-Rosenthal, nageotte, and improved neubauer
• The MAIN DIFFERENCE between the 3 main types of hemocytometer is their DIFFERENT DESIGN of what?

  calibrated counting area
• Give the disadvantages of Manual Cell Count?

  Labor intensive, time consuming, Needs advanced technical skills, poor precision, and subject to error
• What do medtechs need to do to solve the disadvantages brought upon by manual cell counting?

  be TECHNICALLY PROFICIENT
• Automated hemocytometer: has automated cell counting chambers
• Automated cell counting chambers can cause ERRONEOUS RESULTS with LOW CELL COUNTS.
• Normally, the number of RBCs and WBCs in body fluids are what?

  Low
• To SOLVE the problems brought upon by automated hemocytometer, what should be done?

  Define LOWER LIMITS and follow PROTOCOL
• The improved neubauer has how many LARGE COUNTING AREAS?

  9
• What is the size of a whole hemocytometer in mm?

  3mm by 3mm
• What is the size of the individual 9 large counting area?

  1x1 mm2
• Each 9 large counting squares is divided into how many more squares?

  16
• Large squares for RBC counting contains how many more squares?

  100
• The middle part of a hemocytometer for RBC counting has how many more squares?

  400
• Label the following:

  A) Overflow

  B) Sample

  C) Cover glass

  D) Counting grid

  
• What is the area of 1 W square?

  1mm2
• What is the area of the middle R square?

  0.04mm2
• How many squares are used for RBC count?

  5
• How many squares are used for WBC count?

  4