Dilution of Sample

Cards (6)

  • 1st step is to gently mix the test tube containing the sample by inversion at least how many times?
    3 times
  • 2nd step is to attach an ASPIRATOR to the SUCKING end of a THOMA WBC DILUTING PIPETTE and then draw the sample up the 0.5TH MARK in the stem.
  • 3rd step is for any excess samples in the pipette, gently remove them by dispensing the contents in a what?
    clean gauze
  • 4th step is while holding the pipette VERTICALLY, place the TIP of the pipette in the tube containing 2% ACETIC ACID and aspirate until the 11TH MARK.
  • 5th step is to place the pipette HORIZONTALLY and firmly hold the opening at the tip of the pipette using your THUMB. Detach the sucking tube from the other end of the pipette and cover that opening with the INDEX or MIDDLE finger.
  • 6th step is to vigorously mix the sample in the pipette by doing a FIGURE OF 8 motion with your hand for 45 SECONDS.