experiment revison

• To Isolate DNA from a Plant Tissue

  1. Filtration

  2. Blender run for 3 seconds only

  3. Add ice-cold ethanol

  4. Blend mixture

  5. Filter mixture
• Replication


  Making a copy
• Transcription


  RNA production
• Sodium chloride (salt)


  Used to clump the DNA
• DNA


  • White stringy material
• 'Junk' DNA


  Non-coding DNA
• DNA profiling


  Analysing or examining a person's DNA (to compare patterns with others) or getting or providing, patterns or bands
• Chromosome


  Composed of DNA and protein
• Protease enzyme


  Used to break down proteins around the DNA
• To Prepare a Transverse Section of a Dicot Stem

  1. Cut stem safely

  2. Transfer stem section to glass slide

  3. View stem under microscope
• Vascular tissue


  • Transport or Support
• Stem


  • Transport / Support
• Dicotyledonous plants


  • Oak, ash, sycamore, Busy Lizzie, Celery, Begonia
• Tissues in dicot stem

  • Dermal, Ground, Meristematic
• Phloem


  Transport of food (or minerals or auxins)
• Xylem


  Transport of water or minerals
• Evidence that it was a dicot stem: Vascular tissue in a ring shape
• To investigate the growth of leaf yeast using agar plates


  1. Use nutrient medium

  2. Use antiseptic wash solution

  3. Use petroleum jelly

  4. Incubate dishes upside down
• Parasitic fungi


  One group of fungi based on nutrition
• Saprophytic


  The other group of fungi based on nutrition
• Aseptic techniques


  Used to avoid contamination in the lab
• Sterile


  Completely free from microorganisms or living things or named organism
• Yeast


  Part of the Fungi kingdom
• Budding


  Asexual reproduction in yeast
• Nutrient medium


  Malt agar
• Control: The dish with no leaf in it
• Countryside leaf showed growth of leaf yeasts, city leaf showed no growth, the control showed no growth