DNA hybridisation

• dna probes

  short single strand of DNA, complementary to a specific gene sequence/DNA

  • used to locate specific alleles
  • labelled radioactive or fluorescent
• DNA hybridisation

  DNA probes attaches to the complementary DNA base sequence of a gene we are identifying
• process of DNA hybridisation

  amplify the DNA probes - PCR

  heat the DNA sample

  • to break the hydrogen bonds and separate the strands

  add the probes to the DNA sample and cool the mixture

  • allows the complementary base sequences to bind

  sample is washed

  • any DNA probe that aint bound

  can be identified by the radioactive/labelled probe
• uses of genetic screening:

  genetic counselling - identify heritable conditions and risks - change of lifestyle

  personalised medicine - targeted drugs to treat heritable conditions