HISTOPATH

• Periodic Acid Schiff


  Staining of carbohydrates, especially glycogen. May also stain mucoproteins, mucin, carbohydrates, glycoproteins, glycolipids, unsaturated lipids and phospholipids.
• Periodic Acid Schiff


  • Glycogen
  • Mast cell granules
  • Fibrin
  • Mucin
• Langhan's Iodine Method


  • Glycogen
  • Amyloid
• Metachromatic Staining


  • Glycosaminoglycans
• Metachromatic Toluidine Blue


  • Glycosaminoglycans
• Colloidal (Dialyzed) Iron Technique

  • Acid mucin
• Alcian Blue Technique


  • Acid mucopolysaccharide
• Combined Alcian Blue-PAS-hematoxylin Technique

  • Differentiate acid mucin from neutral mucin
• Southgate's Mucicarmine Technique


  • Encapsulated fungi: C. Neoformans
• Gomori's Aldehyde Fuchsin


  • Sulfated mucins
• Mucicarmine Stain


  • Mucin
• Fluorescent Acridine Orange Technique

  • Acid mucin and mucopolysaccharide
• Alcian Blue Stain


  Stains acid mucin blue in colon
• Periodic Acid Schiff


  Used for demonstration of glycogen, especially with diastase method
• Best Carmine Method


  Good affinity for glycogen, stains it bright red. Also weakly stains mast cell granules, fibrin and mucin.
• Langhan's Iodine Method


  Obsolete, not specific for glycogen, rapid but tends to fade
• Metachromatic Staining


  Azure A and Toluidine blue give red-purple stain
• Alcian Blue Technique


  Most popular method for acid mucin, stains it blue
• Combined Alcian Blue-PAS-Hematoxylin Technique

  Neutral mucins stained by PAS, acid mucin stained by Alcian blue
• Gomori's Aldehyde Fuchsin


  Demonstrates sulfated mucins, staining it purple
• Mucicarmine Stain


  Demonstrates mucin by adding aluminum hydroxide to carmine, stains it red
• Colloidal (Dialyzed) Iron Technique

  Absorbed onto tissue containing acid mucin at low pH, visualized by conversion to ferric cyanide
• Fluorescent Acridine Orange Technique


  Gives selective brilliant orange fluorescence, temporary and lasts 2 hours once mounted
• Alkaline Fast Green


  Stains basic proteins like protamines and histones
• Peracetic Acid-Alcian Blue


  Stains cystine and cysteine
• Sakaguchi's Test


  Stains arginine
• Sudan Black


  Stains lipids
• Sudan IV (Scharlach R)

  Stains triglycerides
• Oil Red O


  Stains fats
• Nile Blue Stain


  Stains neutral fats, cholesterin esters, cholesterin fatty acids, cerebrosides, fatty acids, soap
• Osmic Acid Stain


  Stains unsaturated fats
• Toluidine Blue Acetone Method

  Stains sulfatide deposits
• Borohydride Periodic Schiff


  Stains gangliosides
• Perchloric acid-napthoquinone


  Stains cholesterol
• Sudan Black


  Stains lipid droplets in muscle fiber
• Sudan IV


  Intensified and preserved with addition of benzoic acid
• Osmic Acid


  Unstable oxide used as fixative for EM and histochemistry
• Nile Blue Sulfate


  Differentiates lipid by its oxazone; red for neutral lipids, blue for basic lipids
• Toluidine Blue


  Also known as Bodian and Lake
• Gomori - Calcium Method


  Demonstrates alkaline phosphatase activity, gives brownish-black stain