TISSUE ADHESIVE PREPARATION

• Tissue adhesive


  A solution or mixture used to help tissues adhere to glass slides during staining
• Tissue adhesives


  • Not necessary for routine staining
  • Not mandatory in histopathology department
  • Can be essential for routine staining when tissues are fragile and exposed to extreme pH
• Applying tissue adhesive


  1. Spread thinly and evenly on a clean, grease-free slide

  2. Can use fingers, clean foam/rubber sponge, or another slide to spread
• Mayer's egg albumin


  The most commonly used tissue adhesive in the laboratory
• Making Mayer's egg albumin


  Separate egg yolk and white, add 50ml egg white to 50ml glycerin, filter, add 100mg thymol crystals
• Excess albumin takes up acidic stain, so excess should always be wiped from the slide periphery

  
• Glycerin is added to prevent drying and cracking of tissue sections
• Thymol is added to prevent mold growth
• Equipment and materials for making Mayer's egg albumin

  • Filter paper
  • Funnel
  • Stirring rod
  • Thymol
  • Glycerin
  • Egg white