Parasites and Bacteria

• This parasite is known as:

  Ascaris lumbricoides

  • Roundworm

  (largest intestinal roundworm found in man)

  
• This parasite is known as:

  Ancylostoma duodenale

  • Hookworm

  
• This parasite is known as:

  Trichuris trichiura

  • Whipworm

  
• This parasite is known as:

  Strongyloides stercoralis

  • Threadworm

  
• This parasite is known as:

  Enterobius vermicularis

  • Seatworm

  
• This parasite is known as:

  Giardia lamblia cyst

  
• GRAM STAIN

  Procedure (VIAS):

  1. Crystal Violet
  2. Gram's Iodine
  3. Alcohol
  4. Safranin
• GRAM STAIN

  Crystal Violet is used as...

  • Primary Stain
• GRAM STAIN

  Gram's Iodine is used as...

  • Mordant
• GRAM STAIN

  Alcohol is used as...

  • Decolorizer
• GRAM STAIN

  Safranin is used as...

  • Counter stain
• GRAM STAIN (Time)

  • Crystal Violet - 1 min
• GRAM STAIN (Time)

  • Gram's Iodine - 1 min
• GRAM STAIN (Time)

  • Alcohol - 30 secs
• GRAM STAIN (Time)

  • Safranin - 1 min
• GRAM STAIN (Results)

  Crystal Violet

  • Gram (+) = purple
  • Gram (-) = purple
• GRAM STAIN (Results)

  Gram's Iodine

  • Gram (+) = purple
  • Gram (-) = purple
• GRAM STAIN (Results)

  Alcohol

  • Gram (+) = purple
  • Gram (-) = colorless
• GRAM STAIN (Results)

  Safranin

  • Gram (+) = purple
  • Gram (-) = pink or red
• Bacteria is known as...

  • Staphylococcus aureus

  
• Bacteria is known as...

  • Escherichia coli

  
• A gram stain is used to determine the type of bacteria present in a sample.
• Bacteria (SHAPES)

  • Cocci
  • Bacilli
  • Coccobacilli
• Bacteria (SHAPES)

  • Cocci

  
• Bacteria (SHAPES)

  • Bacilli

  
• Bacteria (SHAPES)

  • Coccobacilli

  
• Bacteria (ARRANGEMENT)

  • Singly
  • Pairs
  • Triads
  • Tetrads
  • Clusters
  • Chains
• Bacteria (ARRANGEMENT)

  • Singly

  
• Bacteria (ARRANGEMENT)

  • Pairs

  
• Bacteria (ARRANGEMENT)

  • Triads

  
• Bacteria (ARRANGEMENT)

  • Tetrads

  
• Bacteria (ARRANGEMENT)

  • Clusters

  
• Bacteria (ARRANGEMENT)

  • Chains

  
• Manner of Reporting:

  • Gram (+/-) [Shape] in [Arrangement]
• Parasite:

  • Ascaris lumbricoides ova

  
• Parasite:

  • Trichuris trichiura ova

  
• Parasite:

  • Hookworm (Ancylostoma duodenale)

  
• Parasite:

  • Enterobius vermicularis