Microscopy

• What is a limitation of a light microscope?

  It has a low resolution.
• What is the resolution limitation of a light microscope due to?

  The light rays with long wavelengths.
• How can a light microscope's resolution limitation be overcome and why?

  By using an electron microscope with beams of electrons as they have shorter wavelengths.
• What can an electron microscope do with beams of electrons with shorter wavelengths?

  Distinguish between two objects only 0.1 nm apart.
• What is the equation for magnification?


  Magnification = size of image/size of real object.
• What is the resolution of a microscope?

  The minimum distance apart that two objects can be for them to appear as separate things.
• What does the resolving power depend on in a microscope?

  The wavelength or form of radiation used.
• What is the resolution in a light microscope?

  0.2 micrometers.
• What is the magnification of a microscope?

  The size of the object.