Restriction Endonucleases

• Restriction Endonucleases


  Enzymes that cut up DNA (naturally occur in bacteria as defense mechanism)

  • Numerous restriction enzymes with active site complementary to base sequences, known as recognition sequences, cut at specific locations
• Blunt ends


  When the enzyme cuts at the same location
• Sticky ends


  When the enzyme cuts at staggered ends to expose DNA bases, being palindromic