Immunological comparison of proteins
1. Comparing blood proteins of different species
2. Isolating human blood serum (plasma with the clotting protein removed)
3. Injecting human blood serum into a rabbit
4. Rabbit producing anti human antibodies
5. Adding a sample of the rabbit serum to a sample of human serum
6. Precipitate forming due to antigen antibody complex
7. Adding samples of rabbit serum to serum containing blood proteins from other mammals
8. Comparing the degree of precipitation to that caused by human serum
9. If degree of precipitation is similar to human serum, they are closely related