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BIOCHEMISTRY-LEC
BIOCHEMICAL REACTIONS
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Cards (23)
Biochemical Reactions
Chemical rxs (reactions) that take place inside the cells
all these rxs are enzyme-catalyzed rxs
Ligation
joining of two nucleic acid fragments through the action of an enzyme
Ligation
a fundamental technique in recombinant DNA technology & genetic engineering
TRUE
"The Ligation of different DNA fragments can create new functional units."
Carbocation
a molecule having a carbon atom bearing three bonds and a positive formal charge.
Dehydration Synthesis
release water molecules as byproducts
Hydrolysis
rx in which a water mol is used during the breakdown
Oxidation
process that involves the loss of electrons
Reduction
process where there is a gain of e-
Hydrogenation
chemical rx between molecular hydrogen (H2) and another compound/element
usually in the presence of a catalyst (Ni, Pd, Pt)
Dehydrogenation
removal of hydrogen, usually from an organic mol
Carboxylation
fixation of CO2 by plants, & bicarbonate by animals, plants, and bacteria
Decarboxylation
eliminates a carboxyl group and liberates CO2
Phosphorylation
addition of a phosphoryl (PO3) group to a mol
Dephosphorylation
removal of a phosphate (PO4^3-) group from an org compound by hydrolysis
Ionization
the formation of ions when water is added to a substance
Dissociation
mols separate/split into smaller particles
Neutralization
rx that yields salts and water when equal amts of acids and bases react
Condensation
rx in which two mols combine to form a single mol
Halogenation
introduces one or more halogens into a chemical compound
Saponification
"hydration rx where free hydroxide breaks the ester bonds between fatty acids & glycerol of a triglyceride, resulting in free fatty acids & glycerol."
Free radical
species which has one or more unpaired e-
Intermediate
compounds
compound produced in the course of the chemical rx
not a final product, but used in further rx to produce final product.