RODRI

• Stinct crossbanding


  Dark red crystals of 10-b-carotene
• Mycobacterium marinum


  With cross-barring
• Mycobacterium ulcerans


  No crossbanding
• Mycobacterium terrae complex


  • M. triviale: Rough/dry colonies
  • M. terrae: Smooth colonies
  • M. nonchromogenicum: Smooth to rough colonies; white to buff
• Mycobacterium leprae


  "Cigar-shaped" or "pocket-fence" arrangement
• Clostridium perfringes


  Subterminal spores, "boxcar-shaped"
• Clostridium perfringes culture


  • BAP: Double zone of hemolysis
  • Litmus milk: Stormy fermentation
• Clostridium tetani


  Terminal spores, "drumstick" or "tennis racket" appearance
• Clostridium botulinum


  Subterminal spores
• Clostridium difficile


  Subterminal spores
• Clostridium difficile culture


  • "Ground-glass" appearance in cycloserine-cefoxitin-fructose agar (CFFA)
  • BAP: "Horse stable" odor
• Anaerobic Actinomycetes culture


  • Young colonies: "Spider-like" or "wooly" appearance
  • Old colonies: "Molar tooth" appearance
• Bacteroides fragilis


  Safety pin appearance
• Bacteroides ureolyticus


  "Dog bone" shape
• Bacteroides ureolyticus culture: Pits agar
• Clostridium septicum culture


  "Medusa's head" appearance
• Eubacterium culture


  "Seagull wing" shaped; fluorescent chartreuse color
• Fusobacterium culture


  Medium turns green upon air exposure; "breadcrumb-like" colonies
• Lactobacillus culture


  Pinpoint colonies
• Porphyromonas culture


  Brick red fluorescence
• Prevotella culture


  Brick red fluorescence
• Veilonella parvula culture


  Red fluorescence
• Ehrlichia


  "Mulberries" or morula (Wright-Giemsa stain)
• Mycoplasma hominis culture


  "Fried egg" appearance
• Ureaplasma urealyticum culture


  Dark brownish lumps on A7 or A8 agar
• Treponema pallidum subsp. pallidum

  3 axial filaments; 1 insertion disk
• Borrelia spp.


  15-20 axial filaments; 2 insertion disks
• Leptospira


  Tightly coiled, 2 long axial filaments
• Streptobacillus moniliformis


  "L forms" or "Yeast-like" shape
• Streptobacillus moniliformis culture


  • BAP: "Fried egg" appearance
  • Broth: "Fluff balls/bread crumbs"
• Klebsiella (Calymmatobacterium) granulomatis


  Safety pin appearance; presence of Donovan bodies in mononucleated endothelial cells
• Capnocytophaga culture: Slight yellow or orange pigmentation
• Pinpoint colonies


  • Streptococcus epidermidis
  • Corynebacterium urealyticum
  • Lactobacillus
• Safety pin appearance


  • Yersinia pestis
  • Bacteroides fragilis
  • Klebsiella granulomatis
• Fried egg appearance


  • Corynebacterium diphtheria biotype mitis
  • Mycoplasma hominis
  • Streptobacillus moniliformis
• Medusa head


  • Bacillus anthracis
  • Clostridium septicum
  • Kurthia
• Chinese letters (ALDAVE)


  • C. amylocatum – normal
  • C. minutissimum – normal
  • C. diptheriae – pathogenic
  • C. ulcerans – pathogenic
  • C. pseudotuberculosis – pathogenic
• With bipolar bodies


  • Yersinia pestis
  • Yersinia enterocolitica
  • Buckholderia pseudomallei
  • Francisella tularensis
  • Pasteurella multicoda
• Spreading colonies


  • Proteus
  • Bacillus cereus
  • Campylobacter
  • Clostridium tetani
  • Clostridium septicum
• Pits agar


  • Chromobacterium violaceum
  • Cadiobacterium hominis
  • Eikenella corrodens
  • Arcanobacterium
  • Bacteriodes ureolyticus
  • Moraxella lacunata