Diagnosis of Malaria

• What is the GOLD STANDARD for diagnosis of malaria?

  thin and thick blood smear
• What stain is used for thick and thin blood smear?

  giemsa or wright stain
• What specimen is used for thick and thin blood smear?

  capillary or venous blood
• Capillary or venous blood for thick and thin blood smear is collected BEFORE FEVER strikes or EVERY 6-12 HOURS for up to 48 HOURS (febrile).
• How many smears do you collect for Thick and thin smear blood preparation procedure?

  2 smears per patient
• Complete the labels:

  A) dehemoglobinized blood

  B) 1.5cm2

  C) center of slide

  D) air dry

  E) 100 IOF

  F) RAPID IDENTIFICATION

  
• Complete the label:

  A) feathered edge

  B) air dry

  C) absolute methanol

  D) 200-300 IOF

  E) Species ID

  
• What is the malarial antigen of P. falciparum?

  HRP-2 (histidine-rich protein)
• What are the malarial antigens of all malarial parasites?

  pLDH (parasaite lactate dehydrogenase) and plasmodium aldolase
• What are the 3 tests used for malarial antigens?

  Parasight F, OptiMAL test, and ICT malaria P.f and P.v
• Parasight F detects what malarial antigen?

  Histidine-rich protein 2
• What is the positive result of PARASIGHT F?

  pink line
• Parasight F. detects the presence of what?

  Plasmodium falciparum
• What does OptiMAL test detects?

  HRP-2 and pLDH
• +2 lines: P. vivax in OptiMAL test
• +3 lines: P. falciparum (OptiMAL test)
• principle of ICT malaria?

  immunochromatography
• What does ICT malaria detects?

  HRP-2 and Plasmodium aldolase
• Duffy/Fy (a-b-): resistance to P.vivax
• Sickle cell anemia and G6PD deficiency: resistance to P. falciparum