RP1 - Rate & enzyme-controlled reactions

• Why would a buffer solution be used in this practical?

  To maintain a constant pH (if pH is a control variable)
• Why was it necessary to leave the tets tubes in the water bath for 10 minutes, prior to adding the enzyme to the milk powder?

  To equilibrate solutions & enable temperature to reach reacting temperature
• Explain why you set up 3 experiments at each temperature

  • Enables identification of anomalies
  • Enables calculation of reliable mean (for each temperature)
• How would you set up the control experiment for this practical?

  • Put testing solution & buffer with no enzyme in test tube into water bath. Shows that substrate is hydrolysed by enzyme action & not by temperature