3- DNA Replication

• DNA replication


  1. DNA helicase attaches to DNA molecule

  2. Hydrogen bonds between complementary bases break

  3. Two polynucleotide strands separate

  4. Activated nucleotides line up with complementary bases

  5. DNA polymerase attaches and catalyzes formation of phosphodiester bonds

  6. Two copies of double-stranded DNA molecule formed
• Activated nucleotides


  Nucleotides containing three phosphate groups
• Semi-conservative replication


  Each new DNA molecule contains one strand from the original DNA molecule and one new strand
• Mutation


  Incorrect base inserted into the growing polynucleotide strand, changing the DNA sequence
• 3' end and 5' end


  Ends of polynucleotide strands with hydroxyl and phosphate groups respectively
• DNA polymerase direction


  Copies from 5' end to 3' end of growing strand