describe the structure and function of a cell-surface membrane
cell-surface membranes contain embeddedproteins. they enable control of the passage of substances across exchange surfaces by passive or active transport.
describe the function of the nucleus and name the parts within it
contains the hereditary material and controls the cell's activities. the nucleus is composed of the nuclear envelope, nuclear pores, nucleoplasm, chromosomes and the nucleolus
function and structure of the nuclear envelope
controls the exit and entry of materials in and out of the nucleus, and contains the reactions taking place within it.
a double membrane that surrounds the nucleus.
outer membrane continuous with endoplasmic reticulum and often has ribosomes on its surface.
function and structure of nuclear pores
allows the passage of large molecules out of the nucleus e.g., RNA.
typically around 3000 pores in each nucleus, each 40-100nm in diameter
function and structure of nucleoplasm
granular, jelly-like material that makes up the bulk of the nucleus.
function and structure of chromosomes
consist of protein-bound, linear DNA.
describe the function of mitochondria and name the structures within it
mitochondria are the sites of the aerobic stages of respiration and are responsible for the production of ATP. the structures within are...
double membrane
cristae
matrix
function and structure of double membrane in mitochondria
surrounds the mitochondria and controls the entry and exit of material.
inner of the two membranes is folded to form extensions - cristae.
function and structure of cristae
provide a large surface area for the attachment of enzymes and other proteins involved in respiration.
are extensions of the inner membrane.
function and structure of the matrix
makes up the remainder of the nucleus.
contains proteins, lipids, ribosomes and DNA which allows mitochondria to control the production of their own proteins.
describe the function of chloroplasts and name the main structures within it
chloroplasts are organelles that carry out photosynthesis. chloroplasts compose of...
the chloroplast envelope
grana
stroma
function and structure of the chloroplast envelope
high selective in what it allows to enter and leave the chloroplast.
a double plasma membrane surrounding the organelle.
function and structure of the grana
where the first stage of photosynthesis takes place.
grana are stacks of up to 100 disc like structures (thylakoids) which contain the photosynthetic pigment chlorophyll.
function and structure of the stroma
where the second stage of photosynthesis takes place.
a fluid filled matrix. within the stroma are a number of other structures, such as starch grains
describe the function of the golgi apparatus
process and package lipids and proteins - carried out by the cisternae
store and transport lipids and proteins - carried out by the vesicles
synthesise lysosomes - specialised vesicles
describe the structure of the golgi apparatus
contain fluid-filled membrane bound sacs known as cisternae
contain smaller vesicles
describe the function of lysosomes
digest invading cells - this process uses enzymes
break down waste material - this material includes old organelles
releases hydrolytic enzymes
describe the structure of lysosomes
contain hydrolytic enzymes
surrounded by a membrane to keep enzymes separate from the cytoplasm of the cell
what is the function of ribosomes
site of protein synthesis - involved in the process of translation.
80S found in eukaryotic cells and 70S in prokaryotic cells, mitochondria and chloroplasts.
structure of ribosomes
made up of proteins and rRNA
consist of a large and small subunit
not surrounded by a membrane
function of rough endoplasmic reticulum (RER)
synthesis and transport of proteins
structure of RER
covered with ribosomes (why its called rough)
contains a network of membranes enclosing the cisternae
the surface of the cisternae is covered with ribosomes
function of smooth endoplasmic reticulum (SER)
synthesis, storage and transport of lipids and carbohydrates
structure of SER
same as RER but
lacks ribosomes
function of cell wall
provides strength to prevent the cell bursting under the pressure created by the osmotic entry of water
gives mechanical strength to the plant
structure of cell wall in plants
consists of microfibrils of the polysaccharide cellulose, embedded in a matrix.
a thin layer (middle lamella), which marks the boundary between adjacent cell walls and cements adjacent cells together.
structure of cell wall in algae
made up of either cellulose or glycoproteins, or a mixture of both
cell wall structure in fungi
do not contain cellulose
comprise of chitin - a nitrogen containing polysaccharide, glycan and glycoproteins
function of vacuole in plants
helps to maintain pressure within the cell - keeps the cell rigid and stops the plant from wilting
structure of vacuole in plants
contains cell sap
surrounded by a selectively permeable membrane known as tonoplast
define specialised cells
cells with certain features that allow them to carry out a particular function
define tissue
group of similar cells working together to carry out a particular function
define organ
a group of tissues working together to carry out a particular function
define organ system

a group of organs working together to carry out a particular function
levels of organisations of cells in living organisms and give examples of each

specialised cells - palisade cells
tissues - epithelium, cartilage, and xylem
organs - stomach, pancreas, and leaf
organ systems - cardiovascular system, digestive system, and root system
cell fractionation is a laboratory method used to isolate different components of the cell, specifically the organelles, so they can be studied in detail. it involves breaking the cell open and separating the organelles based on their size and density.
the four main steps in cell fractionation are:
sample preparation
homogenisation
filtration
ultracentrifugation
sample preparation - sample is placed in an ice-cold, isotonic buffered solution to protect the organelles during the fractionation process.
ice-cold - slows down enzyme activity
isotonic - ensures that water potential inside and outside organelles is the same
buffered - keeping the pH constant is crucial to prevent denaturation
homogenisation involves physically breaking open the cells. plasma membrane is disrupted, allowing organelles to be released into the solution.
filtration - mixture is filtered to remove larger pieces of cell debris and any remaining tissue fragments.