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Biology :
B2 : organisation
Food Tests :
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Cards (12)
Food tests
1.
Prepare
food sample
2.
Crush
food with
mortar
and
pestle
3.
Dissolve
in
distilled
water
4.
Filter
solution
Sugars


Small units that all
carbohydrates
are made of
Types of sugars


Reducing
sugars
Non-reducing
sugars
Benedict's test for reducing sugars
1.
Prepare food sample
2.
Transfer 5cm3
to
test tube
3.
Heat
in
75°C water bath
for 5 minutes
4. Add
Benedict's solution
Benedict's test


Tests for
reducing
sugars
Blue
solution indicates
no
reducing sugars
Green
,
yellow
or
brick red
indicates
reducing
sugars
Iodine
test for starch

1. Take
5cm3
of food sample
2. Add
iodine
solution
3.
Shake
gently
Iodine
test

Brown-orange
solution turns
blue-black
if
starch
is present
Biuret test for proteins
1. Take
2cm3
of food sample
2. Add
2cm3
Biuret solution
3.
Shake
gently
Biuret
test

Blue solution turns
pink
or
purple
if
proteins
are present
Sudan
3
test for lipids

1. Take
5cm3
of food sample
2. Add
3
drops
Sudan
3 stain
3.
Shake
gently
Sudan 3 test


Bright
red
layer at top indicates
lipids
present
Emulsion
test

Cloudy
white
colour indicates
lipids
present