Energy from ATP and hydrogen from reduced NADP are passed from light dependent stage to the light independent stage of photosynthesis
Energy and hydrogen are used during the light independent reactions ( known as Calvincycle) to produce complex organic molecules including Carbohydrates such as
starch
sucrose
Cellulose
Thee main steps:
Rubisco catalyzes the fixation of carbon dioxide by combination with a molecule of ribulose bisphosphate (RuBP) a 5 carbon compound, to yield two molecules of glycerate 3-phosphate (GP) a 3 carbon molecule
GP is reduced to triose phosphate (TP) in a reaction involving reduced NADP and ATP
RuBP is regenerated from TP in reactions that use ATP
Carbon fixation:
Carbon dioxide combines with a 5 carbon (5c) sugar known as ribulose bisphosphate (RuBP)
enzyme called rubisco catalyzes this reaction
result 6c compound is unstable and splits in 2
gives two molecules of 3 carbon molecule known as glycerate 3-phosphate (GP)
carbon dioxide has been 'fixed' (removed from external environment and has become part of organic matter of the plant cell)
Glycerate 3-phosplate (GP) is not a carbohydrate but the next step in calvin cycle converts it into one
Reduction of Glycerate 3-phosphate
1. Energy from ATP
2. Hydrogen from reduced NADP
3. Both produced during light-dependent stage of photosynthesis
4. Used to reduce glycerate 3-phosphate (GP) to a phosphorylated 3c sugar known as triose phosphate (TP)
Uses of triose phosphate (TP) molecules
1/6 used to produce useful organic molecules needed by the plant
Condensed to become hexose phosphates (6C) which can be used to produce starch, sucrose, cellulose
Converted to glycerol and glycerate 3-phosphates to fatty acids which joins to form lipids for cell membranes
Used in the production of amino acids for protein synthesis
Regeneration of Ribulose Bisphosphate
5/6 of TP molecules are used to regenerate ribulose bisphosphate (RuBP)
Process requires ATP
Diagram of Calvin cycle
Calvin cycle intermediates
intermediate molecules of the Calvin cycle (such as glycerate 3-phosphate and triose phosphate) are used to produce some amino acids
TP is used to produce:
Hexose phosphates (6c): starch, sucrose or cellulose