measuring cell size

• Cell size


  Can be measured using an eyepiece graticule
• Eyepiece graticule


  • Has a scale ruled on it
• Measuring cell size using eyepiece graticule

  1. Find out the distance measured for each division of the graticule

  2. Use the graticule to measure cells

  3. The distance will be different for each objective
• Calibrating the eyepiece graticule

  1. Use a stage micrometer

  2. Line up one division on eyepiece graticule with fixed point on stage micrometer

  3. Count number of eyepiece graticule divisions that correspond to set measurement on stage micrometer

  4. Calculate distance in micrometres of one division on eyepiece graticule
• Using calibrated eyepiece graticule to measure cell

  1. Width of cell = 12 eyepiece graticule divisions

  2. Real width of cell = 12 x 4.9 μm = 59 μm