MICROPARA LAB

avatar
Created by
Elloiza Borgonia

Subdecks (1)

Cards (49)

  • Culture Media

    Substances used for cultivation of bacteria and related organisms
  • Culture media

    • Ingredients are varied to meet peculiar growth requirements
    • Its components serve as food of bacteria, indicators, inhibitory substance, control pH
  • Classification of Culture media
    • According to physical state
    • According to Composition
    • According to their use
  • Solid media

    Substances mixed with a solidifying agent, gelatin, or albumin matrix. Agar is usually added in concentrations of 2-3%.
  • Semi-solid media

    0.5-1% agar is added.
  • Liquid media

    Do not contain any solidifying substance.
  • Synthetic media

    Whose exact chemical composition of ingredients is known. Example: Ringer's solution, Locke's solution.
  • Non-synthetic media

    Precise composition for some or all of the nutritive elements not definitely known. Example: meat extract broth
  • Living Tissue media

    Living cells present; used for cultivation of Rickettsiae and viruses. Example: HeLa tissue culture medium.
  • Simple media

    Not enriched and where ordinary organisms are easily cultivated. Example: broth or plain agar media.
  • Enriched media

    Include 5% sheep blood agar, enriched chocolate agar, and a number of infusion based media, all of which may be further enriched with additives. Example: blood agar
  • Differential media

    Contains dyes sugars and indicators designed to elicit characteristic biochemical response (usually color) and used to differentiate groups of organisms such as lactose fermenters and non-lactose fermenters.
  • Differential Mildly Selective Media
    Eosin methylene blue agar, MacConkey agar
  • Differential Moderate Selective Media

    Salmonella shigella agar, Hektoen Enteric agar
  • Highly Selective media

    Promotes growth of specific organism. Example: Lowenstein Jensen medium, Campy-blood agar, Bismuth sulfite agar
  • Transport media

    Prolong survival of microorganisms when significant delay occurs between collection and culturing. Example: Cary Blair TM, Amies TM
  • Enrichment media

    For stool cultures where normal flora of the stool are suppressed favoring growth of enteropathogens. Example: Selenite F broth, Tetrationate broth.