biochem

• Personal Protective Equipment (PPE)


  Clean laboratory gown, gloves, face mask, head cap, goggles (if needed)
• Wearing of PPE


  1. Requirement in the laboratory

  2. Only during small group discussion and dry laboratory activities are students allowed not to wear PPE
• Laboratory materials secured by group

  • Scissors
  • Masking tape
  • Permanent marker
  • Liquid detergent
  • Hand towel
  • Rags
  • Five amber-colored bottles
  • Match
  • Fifteen vials (30ml)
  • Five wide-mouth containers (60ml)
  • One plastic storage box (6L)
  • Two plastic tray
  • Garbage bags (small)
  • Five whistles
  • Sticker paper
• Pre-laboratory responsibilities


  1. Read the experiment ahead of time

  2. Research ahead on the Material Safety Data Sheet (MSDS) of every chemical, glass ware and apparatus

  3. Make a schematic diagram of the experiment
• Cleaning of glasswares and apparatus

  1. Using liquid detergent/ cleaning solution before using

  2. Using liquid detergent before returning, dry it prior to returning
• Post-laboratory responsibilities


  1. Properly place all chemicals used in the experiment inside their respective containers

  2. Each group is responsible for the cleanliness of their work area
• Reservation of chemicals


  1. Chemicals should be reserved by the student one meeting prior to the next experiment

  2. Chemicals should be claimed within the first 15 minutes of the laboratory period

  3. Failure to reserve chemicals for an experiment will mean NO EXPERIMENTATION

  4. Check the dispensed chemicals upon receiving and before leaving the Pharmacy stockroom

  5. Complaints after dispensing must be reported to the laboratory personnel immediately
• Borrowing and returning of glasswares and laboratory apparatus

  1. Reservations of the apparatus must be done one meeting before the experiment

  2. Fill out all the necessary information in the borrower slip

  3. Glasswares will be dispensed within the first 15 minutes of the laboratory period

  4. Glasswares breakages or apparatus damages should be reported immediately

  5. The laboratory stockroom will be using the PRIORITY NUMBER/ FIRST COME FIRST SERVE in dispensing reserved glasswares and apparatus

  6. Check the glasswares and apparatus upon receiving and prior to leaving the stockroom

  7. Fifteen minutes before the end of the period, the glasswares and apparatus should be returned to the laboratory dispensary CLEAN AND DRY

  8. Laboratory personnel have the authority to decline the glasswares to be returned, if deemed dirty/damaged
• Rules of breakages


  1. The group will have one week to replace the broken/ damaged apparatus and glasswares

  2. After one week, the amount of the damaged glasswares and apparatus will automatically be charged to the students' account
• Take out apparatus


  1. A take-out apparatus form must be obtained by the students from the laboratory stockroom

  2. Duplicate copy of the take out apparatus form must be secured by the group

  3. The group must specify the date of return for the take out apparatus

  4. In the event of returns beyond the specified date, the cost of the apparatus will be charged to the students' account
• Chemical waste management


  1. Students must endorse the waste product of the experiment to the instructor

  2. Chemical wastes must be placed in the reagent waste bottles

  3. Do not perform unnecessary experimentation with the excess chemicals

  4. Do not discard chemicals in the sink and garbage bins

  5. Reagent bottles will be provided by the laboratory stockroom
• Room care


  1. Upon completion of the activity and returning of the requested apparatus, make sure the working table is cleaned

  2. Garbage must be placed in the garbage bag

  3. Classroom lights and fans must be turned off before leaving the laboratory

  4. Used gloves, mask and head caps must be disposed in the garbage bag
• Laboratory safety protocols


  • The laboratory can be but is not necessarily a dangerous place
  • When intelligent precaution and a proper understanding of techniques are employed, the laboratory is no more dangerous than any other lesson in the classroom
  • Most precautions are just common-sense practices
• Safety rules to be strictly followed by all students

  • Wear your FACE MASK all the time
  • Wear goggles when required
  • DO NOT touch chemicals with your hands
  • DO NOT eat or drink in the laboratory
  • DO NOT taste any chemical
  • DO NOT smell chemicals directly
  • DO NOT pipet solutions using your mouth
  • DO NOT put flammable liquids near open flame
  • When heating liquids, make certain that the open end of the tube is directed away from the students
  • DO NOT sit on the laboratory benches
  • DO NOT engage in any games of horseplay in the laboratory
  • DO NOT pour chemicals into the sink
  • All broken glasswares should be cleaned immediately
  • Do all reactions with noxious or dangerous chemicals in the fume hood
  • If a chemical comes into contact with your skin, wash the area with large quantities of water
  • When pouring one liquid to another, do it slowly and cautiously
  • All laboratory experiments should be done under the supervision of the instructor
  • Exercise good housekeeping practices in the laboratory
  • BE CAUTIOUS and ALWAYS THINK WHAT YOU ARE DOING
  • Know where to find and how to use safety and first-aid equipment
  • Wearing of closed shoes in the laboratory is a requirement
  • DO NOT perform any unauthorized experiments in the laboratory
  • Avoid rubbing your eyes within the laboratory
  • In cases of breakages, notify the faculty and the laboratory personnel right away
• Personal Protective Equipment (PPE)


  Clean laboratory gown, gloves, face mask, head cap, goggles (if needed)
• Wearing of PPE


  1. Required in the laboratory

  2. Not required during small group discussion and dry laboratory activities
• Laboratory materials secured by group

  • Scissors
  • Masking tape
  • Permanent marker
  • Liquid detergent
  • Hand towel
  • Rags
  • Five amber-colored bottles
  • Match
  • Fifteen vials (30ml)
  • Five wide-mouth containers (60ml)
  • One plastic storage box (6L)
  • Two plastic tray
  • Garbage bags (small)
  • Five whistles
  • Sticker paper
• Pre-laboratory responsibilities


  1. Read the experiment ahead of time

  2. Research on Material Safety Data Sheet (MSDS)

  3. Make a schematic diagram of the experiment
• Cleaning of glasswares and apparatus

  1. Using liquid detergent/ cleaning solution before using

  2. Drying before returning
• Post-laboratory responsibilities


  1. Clean glasswares and apparatus using liquid detergent before returning

  2. Properly place chemicals in respective containers

  3. Ensure cleanliness of work area
• Reservation of chemicals


  1. Reserve chemicals one meeting prior

  2. Fill up chemical reservation slip

  3. Claim chemicals within first 15 minutes of lab period

  4. No reservation means no experimentation
• Borrowing and returning of glasswares and apparatus

  1. Reserve apparatus one meeting before

  2. Request using laboratory borrower slip

  3. Submit labelled laboratory baskets

  4. Glasswares dispensed within first 15 minutes

  5. Report breakages/damages immediately

  6. Return glasswares 15 minutes before end of period
• Rules of breakages


  1. Replace broken/damaged apparatus and glasswares within one week

  2. After one week, cost charged to student account
• Take out apparatus


  1. Obtain take-out apparatus form

  2. Specify date of return

  3. Cost charged for late returns
• Chemical waste management


  1. Endorse waste to instructor

  2. Place in reagent waste bottles

  3. Do not discard in sink or garbage
• Room care


  1. Clean working table

  2. Dispose garbage

  3. Turn off lights and fans

  4. Dispose used PPE
• Laboratory safety


  • Intelligent precaution and proper understanding of techniques are required
  • Assume all substances are toxic and handle accordingly
  • Be familiar with safety equipment
• Safety rules


  • Wear face mask at all times
  • Wear goggles when required
  • Do not touch chemicals with hands
  • Do not eat or drink in lab
  • Do not taste chemicals
  • Do not smell chemicals directly
  • Do not pipet with mouth
  • Do not put flammable liquids near open flame
  • Be cautious when heating liquids
  • Turn off Bunsen burner when finished
  • Do not sit on benches
  • Do not engage in horseplay
  • Do not pour chemicals in sink
  • Clean up broken glasswares immediately
  • Do reactions with noxious chemicals in fume hood
  • Wash skin if chemical contact
  • Pour acid into water, not water into acid
  • Experiments under instructor supervision
  • Practice good housekeeping
  • Be cautious and think
  • Know location and use of safety equipment
  • Wear closed shoes
  • Do not perform unauthorized experiments
  • Avoid rubbing eyes
  • Notify faculty and lab personnel of breakages
• Beaker


  Cylindrical container used to store, mix and heat liquids in laboratories
• Erlenmeyer flask


  Also known as a conical flask or titration flask, the slanted sides and narrow neck allow the contents to be mixed by swirling without risk of spillage, making them suitable for titrations
• Graduated Cylinder


  Piece of laboratory glassware used to measure the volume of liquids, used to accurately measure the volume of chemicals for use in reactions
• Test tube


  Hold, mix or heat small quantities of liquid or solid chemicals for assays and qualitative experiments, also serve as temporary storage for chemicals
• Watch Glass


  Circular, slightly-concave pieces of glass used by chemists to evaporate liquids and cover beakers during sample preparation
• Burette


  Volumetric measuring glassware used in analytical chemistry for the accurate dispensing of a liquid, especially of one of the reagents in a titration
• Laboratory Funnel


  Channel liquids or fine-grained substances into containers with a small opening
• Crucible with cover

  Small container used to hold reactants during heating at higher temperatures
• Stirring rod


  Piece of laboratory equipment used to mix chemicals and liquids for laboratory purposes
• Distilling Flask


  Piece of laboratory equipment used to separate mixtures of two liquids with different boiling points
• Cuvette


  Piece of laboratory equipment intended to hold samples for spectroscopic analysis
• Laboratory casserole


  Used for melting, ashing or drying out samples