Spectrophotometer

• Types of applications/uses of spectrophotometers

  • Quantitative analysis
  • Quality control
  • Molecular biology
• Definition

  Measures the intensity of transmitted, reflected or emitted light at different wavelengths, producing a spectrum
• Principle of spectrophotometry

  Beer's Law and Lambert's Law
• Advantages of spectrophotometry

  • High sensitivity
  • Accuracy
  • Non-destructive measurement
  • Rapid analysis
• Spectrophotometers are expensive
• Types of spectrophotometer

  • Single-beam
  • Double-beam
  • UV spectrophotometer