CP4: Effect of enzymeconconinitialratesof reaction

Cards (1)

  • Devise a method to measure effect of enzyme conc on initial rate of reaction
    IV = No. of potato discs (5-25)
    DV = Vol of O2 produced
    CV = Same SA of discs, temp(water bath), pH (buffer), same amount of time to measure change in vol, vol + conc of excess H2O2,
    Method:
    • Use cork borer to get potato peices
    • Cut discs of same size - ruler + scalpel
    • Dry with absorbent paper towel
    • Put 5 into a test tube
    • Put in water bath at 30°C for 5 mins
    • Record initial vol, in inverted measuring cylinder
    • Add 20cm3 10% excess H2O2 into test tube
    • Immediately place bung with attached delivery tube going inside inverted measuring cylinder
    • Start stop watch when first bubble of O2 is seen
    • Measure vol every 30 secs, for 5 mins
    • Repeat 2 more times
    • Repeat for each no. of discs
    • Plot a vol/time graph for each no. of potato dics
    • Use tangent to find initial rate
    • Plot initial rate graph