-> ionic, hydrogen, disulphide bridge and hydrophobic regions
Effect of substrate concentration 1:
When enzyme concentration is low, low numbers of substrate enzyme complexes are made per second, low rate of reaction
Effect of substrate concentration 2:
As substrate concentration increases, number of enzyme substrate complexes made are increased per second due to more frequent collisions between enzyme and substrate. Rate of reaction increases almost reaching a maximum
Effect of substrate concentration 3:
Substrate concentration is saturated, number of enzyme and substrate collisions have reached a maximum frequency, reached maximum number of enzyme substrate complexes per second, reached maximum ROR. More substrate no longer impacts rate
Competitive inhibitors
-Structure and chemical properties are similar to the shape of the substrate therefore they compete with the substrate for the right to bind to the active site
-enzyme will bind to the competitive inhibitor temporarily so will be available for substrate breakdown again
-the effect of the competitive inhibitor diminishes as substrate conc. increases due to the likelihood of a collision
Non-competitive inhibitor
Binds to the allosteric site and binds irreversibly
Non competitive inhibitors
Do not exist in direct competition to the substrate
Increasing substrate concentration will not effect the level of inhibition
