microscopy

• What does microscopy refer to?


  The use of microscopes
• What are the main components of a light microscope?


  Base, arm, light source, stage, objective lenses, eyepiece lens, body tube, focusing knobs
• What is the function of the base in a light microscope?


  It supports the entire microscope
• What is the purpose of the arm in a light microscope?


  It connects the base to the top of the microscope
• What type of light source can be used in a light microscope?


  A lamp or a mirror that reflects room light
• Where is the stage located on a light microscope?


  Just above the light source
• What are objective lenses in a light microscope?


  They are lenses with different magnifications used to view the object
• How many objective lenses are typically found in a light microscope?


  Three
• What are the typical magnifications of objective lenses?


  10 times, 20 times, and 50 times
• What is the eyepiece lens in a light microscope?


  A lens with a fixed magnification located at the top
• What are the coarse and fine focusing knobs used for?


  To help get the image in focus
• What does the term 'object' refer to in microscopy?


  The real object or sample being observed
• What does the term 'image' refer to in microscopy?


  The visual representation seen when looking through the microscope
• How does light travel through a light microscope?


  It reflects off the mirror, passes through the object, and through the lenses into the eye
• What is magnification in microscopy?


  How many times larger the image is than the object
• What is the equation for magnification?


  Magnification = image size / object size
• What is resolution in microscopy?


  The shortest distance between two points that can still be distinguished as separate entities
• How does resolution affect the clarity of an image?


  Higher resolution allows for more detail and less blurriness
• What can be inferred from two images of onion cells with the same magnification but different resolutions?


  The image with lower resolution appears blurrier and has less detail
• What are the key differences between object and image in microscopy?


  • Object: The real sample being observed (e.g., onion cells)
  • Image: The visual representation seen through the microscope
• What is the process of light travel in a light microscope?


  1. Light hits the mirror and reflects upwards
  2. Light passes through the object on the stage
  3. Light goes through the objective lens
  4. Light passes through the eyepiece lens
  5. Light enters the eye
• What is the relationship between magnification and resolution?


  • Magnification: Size of the image relative to the object
  • Resolution: Clarity and detail of the image
  • Higher magnification does not guarantee higher resolution