microscopy

• What is microscopy?


  Microscopy refers to the use of microscopes.
• What are the main components of a light microscope?


  The main components include the base, arm, light source, stage, objective lenses, eyepiece lens, body tube, and focusing knobs.
• What supports the entire light microscope?


  The base supports the entire light microscope.
• What connects the base to the top of the microscope?


  The arm connects the base to the top of the microscope.
• What is the function of the light source in a light microscope?


  The light source illuminates the object being viewed, either through a lamp or a mirror reflecting room light.
• Where is the stage located on a light microscope?


  The stage is located just above the light source.
• How many objective lenses are typically found in a light microscope?


  Typically, there are three objective lenses with different magnifications.
• What are the common magnifications of objective lenses in a light microscope?


  Common magnifications are 10 times, 20 times, and 50 times.
• What is the purpose of the eyepiece lens in a light microscope?


  The eyepiece lens has a fixed magnification and is where the viewer looks into the microscope.
• What are the coarse and fine focusing knobs used for in a light microscope?


  They are used to help get the image in focus.
• What does the term 'object' refer to in microscopy?


  The term 'object' refers to the real object or sample being observed, such as onion cells on a slide.
• What does the term 'image' refer to in microscopy?


  The term 'image' refers to the visual representation seen when looking through the microscope.
• How does light travel through a light microscope to reach the viewer's eye?


  Light hits the mirror, reflects upwards through the object, passes through an objective lens, then through the eyepiece lens into the eye.
• What is magnification in the context of microscopy?


  Magnification is how many times larger the image is than the object.
• What is the equation for calculating magnification?


  Magnification is equal to image size divided by object size.
• What is resolution in microscopy?


  Resolution is the shortest distance between two points on an object that can still be distinguished as separate entities.
• How does resolution affect the detail of an image?


  A higher resolution allows for more details to be seen and results in a less blurry image.
• If two images of onion cells have the same magnification but one appears blurrier, what does this indicate about their resolution?


  The blurrier image has a lower resolution.
• What are the key differences between the terms 'object' and 'image' in microscopy?


  • Object: The real sample being observed (e.g., onion cells).
  • Image: The visual representation seen through the microscope.
• What is the relationship between magnification and resolution in microscopy?


  • Magnification: How many times larger the image is than the object.
  • Resolution: The ability to distinguish two separate points; higher resolution means more detail.
• What is the process of light travel in a light microscope?

  1. Light hits the mirror.
  2. Light reflects upwards through the object.
  3. Light passes through the objective lens.
  4. Light goes through the eyepiece lens into the eye.
• What are the functions of the different parts of a light microscope?


  • Base: Supports the microscope.
  • Arm: Connects the base to the top.
  • Light source: Illuminates the object.
  • Stage: Holds the microscope slide.
  • Objective lenses: Provide different magnifications.
  • Eyepiece lens: Where the viewer looks.
  • Focusing knobs: Adjust the focus of the image.