phs effect on enzyme action

• What is the purpose of investigating the effect of pH on enzyme activity?

  To understand how different pH levels influence the activity of enzymes
• What enzyme is used to break down starch in this investigation?

  Amylase
• What does amylase break down starch into?

  Maltose
• How can starch be detected in this experiment?

  Using iodine solution
• What are the steps to set up the experiment to investigate the effect of pH on enzyme activity?

  1. Put a drop of iodine solution into every well of a spotting tile.
  2. Place a Bunsen burner on a heatproof mat with a tripod and gauze.
  3. Heat water in a beaker to 35 degrees Celsius.
  4. Use a syringe to add 3 cm³ of amylase solution and 1 cm³ of buffer solution (pH 5) to a boiling tube.
  5. Place the boiling tube in the heated water.
  6. Add 3 cm³ of starch solution to the boiling tube and mix.
  7. Use continuous sampling to measure the time taken to break down starch.
  8. Repeat with different pH buffers.
• What temperature should the water be heated to in this experiment?

  35 degrees Celsius
• How much amylase solution is added to the boiling tube?

  3 cm³
• What is the pH of the buffer solution used in the experiment?

  5
• What should be done immediately after adding starch solution to the boiling tube?

  Mix the contents
• Why is continuous sampling used in this experiment?

  To measure how long it takes to break down the starch
• What should be done after measuring the effect of pH on enzyme activity with one buffer?

  Repeat the experiment with different pH buffers