Amylase - Required Practical

• Aim: To investigate the effect of pH on the rate of reaction of amylase
• Independent Variable - pH
• Dependent Variable - Time taken for starch to be broken down
• Control - Temp, volume/conc. of starch/amylase
• Rate of Reaction - Divide 1 by the time taken for the reaction in seconds. The calculation is therefore 1/t
• A colorimeter can be used to measure the progress of the reaction more accurately; with a solution containing starch being darker and glucose light (as a result of the colour change of iodine) – this will affect the absorbance or transmission of light in a colorimeter