CC LAB

• What is the title of the document provided?

  Laboratory Safety and Regulations
• Who is the instructor for the course?

  Prof. Kimberly Ann M. Pulga, RMT
• What is the date of the session mentioned in the document?

  September 18, 2021
• What are the main topics covered in the outline of the document?

  • Safety awareness for Clinical Laboratory Personnel
  • Types of Safety Hazards
  • General Laboratory Rules
• What is the shared responsibility for safety in the laboratory?

  The employer and the employee share safety responsibility.
• What is the role of laboratory supervisors in safety management?

  They reflect the attitudes of management toward safety and are essential members of the safety program.
• What is one of the employee's responsibilities regarding safety?

  Know and comply with the established laboratory work and safety methods.
• What should employees do if they notice unsafe conditions?

  Give prompt notification of unsafe conditions or practices to the immediate supervisor.
• What is one of the employer's responsibilities in the laboratory?

  Provide safety information, training, personal protective equipment, and medical surveillance to employees.
• What does OSHA stand for?

  Occupational Safety and Health Administration
• What is the goal of the Occupational Safety and Health Act?

  To provide all employees with a safe work environment.
• What does the Bloodborne Pathogens standard apply to?

  All exposure to blood or other potentially infectious materials in any occupational setting.
• What is the purpose of Universal Precaution?

  To treat all human blood, tissue, and most fluids as if known to be infectious for HIV, HBV, and other bloodborne pathogens.
• What is the Hazard Communication Standard also known as?

  OSHA HazCom Standard
• What does the Occupational Exposure to Hazardous Chemicals standard require?

  Appointment of a chemical hygiene officer and development of a chemical hygiene plan.
• What are some examples of safety equipment required in the laboratory?

  Safety showers, eyewash stations, fire extinguishers, blankets, spill kits, first aid supply.
• What are the types of safety hazards mentioned in the document?

  • Biological Hazard
  • Sharp Hazard
  • Chemical Hazard
  • Radioactive Hazard
  • Electrical Hazard
  • Fire/Explosion Hazard
  • Physical Hazard
• What is a biological hazard?

  It is the harmful microorganism.
• What is the primary method of infection transmission in the laboratory?

  Hand contact.
• What should laboratory personnel do after removing gloves?

  Always wash hands.
• What should sharps be disposed of in?

  Puncture-resistant containers.
• What should be done in case of a chemical spill?

  Flush the area with water for 15 minutes and immediately seek medical attention.
• What is the purpose of the Material Safety Data Sheet (MSDS)?

  To provide information on physical and chemical characteristics, fire and explosion potential, reactivity potential, health hazards, and emergency first aid procedures.
• What does the HAZARDS-IDENTIFICATION SYSTEM inform about?

  It informs firefighters of the hazards they may encounter inside the laboratory.
• What are the essential elements to begin a fire?

  Fuel, heat/ignition source, oxygen, and a reaction chain.
• What should never be done in the laboratory?

  Never eat, drink, smoke, or chew gum in the laboratory.
• What are the types of fires and their corresponding extinguishing materials?

  • Class A: Wood, paper, clothing - Extinguishing Material: Water
  • Class B: Flammable organic chemicals - Extinguishing Material: Dry chemicals, carbon dioxide, foam
  • Class C: Electrical - Extinguishing Material: Dry chemicals, carbon dioxide
  • Class D: Combustible metals - Extinguishing Material: None
  • Class K: Grease, oil, fats - Extinguishing Material: Class K, Class A, B, and C fire extinguishers
• What is the purpose of pipets in the laboratory?

  To transfer specific amounts of liquid from one container to another.
• What is the typical volume range for pipets?

  Between 1 and 100 milliliters.
• What are the classifications of pipets based on design?

  • To contain (TC)
  • To deliver (TD)
• What are the classifications of pipets based on drainage?

  • Blow out
  • Self-draining
• What are the classifications of pipets based on use?

  • Measuring/Graduated
  • Volumetric/Transfer
• What is the meniscus in relation to pipetting?

  The meniscus is the curve at the surface of a liquid in a container, which is important for accurate measurement.
• What should be done to ensure accurate pipetting?

  Hold the pipet vertically and place the tip against the side of the container.
• What happens to the fluid in a pipet after dispensing?

  A small amount of fluid will remain in the tip of the pipet.
• What is an example of a type of transfer pipet?

  Mohr, serologic, volumetric transfer pipets.
• What is the significance of the continuous etched ring on a pipet?

  It indicates the maximum fill line for accurate measurement.
• What are the two main classifications of pipets based on design?

  To contain (TC) and to deliver (TD)
• What are the two drainage classifications of pipets?

  Blow out and self-draining
• What are the two uses of pipets based on their classification?

  Measuring/graduated and volumetric/transfer