Microscopy

• Light microscopes use what to magnify?

  Use light and lenses.
• Electron microscopes use what to magnify?

  Use electrons to form Image
• Adv/Disadv of Light Microscope

  -Cheaper, doesn't cost much to run

  -Can only see large sub-cellular structures like nuclei
• Adv/Disadv of Electron Microscope

  expensive ( to buy and to run)

  Have high magnification

  Have higher resolution

  can see small sub-cellular structures
• What is the Magnification Calculation?

  Magnification = Image Size/Real Size
• Light microscopes are

  Fairly cheap

  see outline of cells

  see large sub-cellular structure

  low resolution
• Electron microscopes are

  Very expensive to buy keep and maintain

  can see small sub cellular structures

  uses electrons to magnify

  higher resolution from higher resolving power
• magnification equation is

  Image size/ object size

  Image

  mag[]object