Dna extraction

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Created by
sntiger

Cards (10)

  • Why are strawberries suitable for DNA extraction?
    They can have up to eight copies of each chromosome
  • What visible form does the extracted DNA take?
    White threads
  • What is the method for extracting DNA from strawberries?
    1. Crush the strawberry in a sealed plastic bag for 2 minutes.
    2. Mix 10 cm³ of washing up liquid, 1 g of salt, and 100 cm³ of water in a beaker.
    3. Add 10 cm³ of the extraction liquid to the strawberry and mix for 1 minute.
    4. Filter the mixture.
    5. Pour 10 cm³ of ice-cold 90% ethanol into the mixture without stirring.
    6. Observe the formation of a white cloudy substance and pull out the DNA with a lolly stick.
  • What is the role of washing up liquid in the DNA extraction process?
    It breaks down cell membranes and releases the DNA
  • How long should the strawberry be crushed in the plastic bag?
    About 2 minutes
  • What is the volume of the extraction liquid added to the strawberry mixture?
    10 cm³
  • What should you do after adding the ethanol to the strawberry mixture?
    Do not mix or stir
  • What happens to the DNA when ethanol is added to the strawberry mixture?
    The DNA separates out into a layer
  • What tool is used to pull strands of DNA from the top layer?
    A lolly stick
  • What should you observe after adding ethanol to the mixture?
    A white cloudy substance forming in the clear layer