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BIOLOGY
BIOLOGY Core practical
Dna extraction
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Cards (10)
Why are strawberries suitable for DNA extraction?
They can have up to
eight
copies of each
chromosome
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What visible form does the extracted DNA take?
White
threads
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What is the method for extracting DNA from strawberries?
Crush the strawberry in a sealed plastic bag for 2 minutes.
Mix 10 cm³ of washing up liquid, 1 g of salt, and 100 cm³ of water in a beaker.
Add
10
cm³ of the extraction liquid to the strawberry and mix for 1 minute.
Filter the mixture.
Pour 10 cm³ of ice-cold 90%
ethanol
into the mixture without stirring.
Observe the formation of a white cloudy substance and pull out the DNA with a
lolly
stick.
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What is the role of washing up liquid in the DNA extraction process?
It breaks down
cell
membranes
and
releases
the
DNA
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How long should the strawberry be crushed in the plastic bag?
About
2
minutes
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What is the volume of the extraction liquid added to the strawberry mixture?
10
cm³
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What should you do after adding the ethanol to the strawberry mixture?
Do
not
mix
or
stir
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What happens to the DNA when ethanol is added to the strawberry mixture?
The DNA
separates
out into a
layer
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What tool is used to pull strands of DNA from the top layer?
A
lolly
stick
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What should you observe after adding ethanol to the mixture?
A
white
cloudy
substance
forming in the
clear
layer
View source
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