1.1 The world of the mircoscope

Cards (28)

  • What should you know after studying microscopy techniques?
    Development of microscopy techniques over time
  • What is the difference in magnification between light and electron microscopes?
    Light microscopes magnify up to 2000x
  • How do you calculate the magnification of a specimen?
    Multiply eyepiece lens by objective lens
  • What is the conversion factor from kilometres to metres?
    1 km = 1000 m
  • How many centimetres are in a metre?
    100 cm
  • How many millimetres are in a centimetre?
    10 mm
  • How many micrometres are in a millimetre?
    1000 µm
  • How many nanometres are in a micrometre?
    1000 nm
  • What is the significance of the first light microscopes developed in the mid 17th century?
    They are still widely used to observe cells
  • What is the maximum magnification of the best light microscopes?
    About 2000 times (2000x)
  • What is the main advantage of light microscopes?
    They can magnify live specimens
  • When was the electron microscope invented?
    In the 1930s
  • What type of images do transmission electron microscopes produce?
    2D images with high magnification
  • What is the main function of scanning electron microscopes?
    To produce 3D images of specimens
  • What is a disadvantage of electron microscopes?
    They are large and very expensive
  • What is the formula to calculate magnification using a light microscope?
    Eyepiece magnification × Objective magnification
  • If the eyepiece lens is 4x and the objective lens is 10x, what is the overall magnification?
    40x
  • How do you label drawings made using a microscope?
    Indicate the magnification used for viewing
  • What is the formula for calculating the size of an object under a microscope?
    Size of image / Magnification = Size of real object
  • If the magnification is x60 and the image size is 1 mm, what is the size of the real object?
    0.0166 mm or 16.6 µm
  • What is the average height of a person when magnified by a light microscope?
    About 1.1 km
  • What is the average height of a person when magnified by an electron microscope?
    About 1000 km
  • What is resolution in microscopy?
    The ability to distinguish two separate points
  • What is the highest resolution power of a light microscope?
    About 200 nm
  • What is the highest resolution power of a scanning electron microscope?
    About 15 nm
  • What is the resolving power of an electron microscope?
    Around 0.2 nm
  • What are the key points about light and electron microscopes?
    • Light microscopes:
    • Magnify up to 2000x
    • Resolving power of about 200 nm
    • Electron microscopes:
    • Magnify up to 200,000x
    • Resolving power of around 0.2 nm
  • What is the formula for magnification?
    Magnification = Size of image / Size of real object