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B1 Cell structure and transport
1.1 The world of the mircoscope
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Cards (28)
What should you know after studying microscopy techniques?
Development
of microscopy techniques over time
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What is the difference in magnification between light and electron microscopes?
Light microscopes
magnify up to
2000x
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How do you calculate the magnification of a specimen?
Multiply
eyepiece lens
by
objective lens
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What is the conversion factor from kilometres to metres?
1 km =
1000
m
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How many centimetres are in a metre?
100
cm
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How many millimetres are in a centimetre?
10
mm
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How many micrometres are in a millimetre?
1000
µm
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How many nanometres are in a micrometre?
1000
nm
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What is the significance of the first light microscopes developed in the mid 17th century?
They are still widely used to observe
cells
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What is the maximum magnification of the best light microscopes?
About 2000 times (
2000x
)
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What is the main advantage of light microscopes?
They can magnify
live specimens
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When was the electron microscope invented?
In the 1930s
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What type of images do transmission electron microscopes produce?
2D
images with high
magnification
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What is the main function of scanning electron microscopes?
To produce
3D
images of specimens
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What is a disadvantage of electron microscopes?
They are large and very
expensive
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What is the formula to calculate magnification using a light microscope?
Eyepiece magnification
×
Objective magnification
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If the eyepiece lens is 4x and the objective lens is 10x, what is the overall magnification?
40x
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How do you label drawings made using a microscope?
Indicate the
magnification
used for viewing
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What is the formula for calculating the size of an object under a microscope?
Size of image
/
Magnification
= Size of real object
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If the magnification is x60 and the image size is 1 mm, what is the size of the real object?
0.0166
mm or
16.6
µm
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What is the average height of a person when magnified by a light microscope?
About
1.1 km
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What is the average height of a person when magnified by an electron microscope?
About
1000 km
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What is resolution in microscopy?
The ability to distinguish two
separate
points
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What is the highest resolution power of a light microscope?
About
200
nm
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What is the highest resolution power of a scanning electron microscope?
About
15 nm
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What is the resolving power of an electron microscope?
Around 0.2
nm
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What are the key points about light and electron microscopes?
Light microscopes
:
Magnify up to
2000x
Resolving power of about
200 nm
Electron microscopes:
Magnify up to
200,000x
Resolving power of around
0.2 nm
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What is the formula for magnification?
Magnification =
Size of image
/
Size of real object
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