Factors affecting enzymes activity

• What are the main factors affecting enzyme activity?

  Temperature and substrate concentration
• What is an enzyme?

  A biological catalyst that speeds up reactions
• What is the active site of an enzyme?

  The region where substrate binds to the enzyme
• Name two types of enzyme inhibitors.

  Competitive and non-competitive inhibitors
• How does a competitive inhibitor work?

  It competes with substrate for the active site
• How does a non-competitive inhibitor affect enzyme activity?

  It changes the shape of the active site
• What is the difference in substrate concentration effects between competitive and non-competitive inhibitors?

  Competitive inhibitors can be overcome by increasing substrate
• How does temperature affect enzyme activity?

  Increased temperature generally increases activity until denaturation
• How does pH affect enzyme activity?

  Extreme pH levels can denature enzymes
• What are two factors that affect enzyme activity?

  • Temperature
  • Substrate concentration
• What is the induced fit model?

  Enzyme changes shape to fit the substrate
• What should you learn for the exam regarding enzyme inhibitors?

  • Definitions of competitive and non-competitive inhibitors
  • Effects on enzyme activity rates
  • Examples in answers
• What factors affecting enzyme activity should be discussed in the exam?

  • Optimum temperature
  • Optimum pH
  • Effects of denaturation
  • Include diagrams in answers
• What should you practice for the exam regarding enzyme inhibition?

  • Drawing and labeling diagrams of inhibitors
  • Graphs showing substrate concentration effects