Use a sterile pipette or wire hoop to transfer bacteria from broth to agar plate (petri dish containing agar jelly).
Spread bacteria evenly over plate using a sterile plastic spreader.
Use sterile forceps to place a multi disc antibiotic ring on the plate. Ringshould only be moved by holding the centre, NOT the arms.
Lightly tape a lid on, invert and incubate at 25°C for 48 hours.don't tape around the entire dish as this prevents oxygen entering and so promotes the growth of more harmful anaerobic bacteria.
