CCHM

• What are pipets used for in the laboratory?

  To transfer specific amounts of liquid
• What is the typical volume range for pipets?

  1 to 100 milliliters
• How are pipets classified based on design?

  • To Contain (Tc)
  • To Deliver (Td)
• How are pipets classified based on drainage?

  • Blow Out
  • Self-Draining
• How are pipets classified based on use?

  • Measuring / Graduated
  • Volumetric / Transfer
• What does a To Contain (Tc) pipet do?

  Contains a volume but does not dispense it
• What is a characteristic of a To Deliver (Td) pipet?

  Dispenses the indicated volume of liquid
• How should a To Deliver pipet be held during use?

  Held vertically against the container's side
• What is a Blow Out pipet characterized by?

  Etched ring near the top of the pipet
• What is a Self-draining pipet designed to do?

  Allow contents to drain by gravity
• What is the difference between Mohr and Serological pipettes?

  Mohr graduations end before the tip
• What is the purpose of an Ostwald-Folin pipet?

  Used for transferring viscous fluids
• What is a Volumetric pipet used for?

  Delivering a single specific volume of liquid
• What is a Pasteur pipet used for?

  Transferring solutions without specific volume
• What are the types of automatic pipets?

  1. Air Displacement
  2. Positive-displacement
  3. Dispenser and dilutor/dispenser
• How does an Air Displacement pipet operate?

  Uses a piston for suction creation
• What is a characteristic of a Positive-displacement pipet?

  Piston moves in the pipet tip like a syringe
• What is the function of a Blood Transfer Device?

  Transfers blood without using syringe needle
• What is the purpose of a tourniquet during venipuncture?

  Helps locate patients' veins easily
• What is the primary antiseptic used in routine phlebotomy?

  70% isopropyl alcohol
• What are the steps in the venipuncture procedure using a syringe?

  1. Obtain requisition form
  2. Greet and reassure patient
  3. Identify patient verbally
  4. Verify fasting and allergies
  5. Select correct tubes and equipment
  6. Wash hands and apply gloves
  7. Position patient's arm
  8. Apply tourniquet and locate vein
  9. Clean site with alcohol
  10. Assemble equipment
  11. Reapply tourniquet and inspect needle
• What should be done after cleaning the venipuncture site?

  Assemble the equipment as alcohol dries
• Why should the patient’s arm be positioned slightly bent during venipuncture?

  To allow tubes to fill from the bottom up
• What should be done after inserting the needle into the vein?

  Blood should appear in the hub of the needle
• What is the purpose of using a gauze pad after venipuncture?

  To apply pressure to the puncture site
• What should be avoided when applying pressure to the puncture site?

  Using cotton balls to apply pressure
• What are the types of thermometers mentioned?

  1. Liquid-in-glass
  2. Electronic thermometer
  3. Digital thermometer
• What are the types of liquid-in-glass thermometers?

  1. Partial Immersion
  2. Total Immersion
  3. Surface Thermometers
• What is the advantage of using an electronic thermometer?

  Size and millisecond response time
• What is the disadvantage of using an electronic thermometer?

  It is expensive
• What is the purpose of a needle disposal system?

  To safely dispose of used needles
• What is the structure of a multi-sample needle?

  Covered by a rubber sheath for safety
• What is the purpose of a phlebotomy collection tray?

  To organize phlebotomy equipment
• What is the role of a phlebotomy drawing station?

  Provides a comfortable area for patients
• What is the purpose of a tourniquet during venipuncture?

  To make veins easier to locate
• What is the purpose of using 70% isopropyl alcohol in phlebotomy?

  To cleanse the skin before venipuncture
• What should be done after blood collection?

  Transfer blood to appropriate evacuated tubes
• What is the purpose of a bandage after venipuncture?

  To protect the puncture site
• What is the purpose of an indelible pen in venipuncture?

  To label blood samples accurately
• What is the purpose of a biohazard container?

  To safely dispose of hazardous materials