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microbiology
why bacteria stain differently
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sophie w
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Cards (17)
what is the function of the
gram stain technique
it is used by
microbiologists
to distinguish between two
types
of bacteria
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what two types of bacteria is the
gram stain
technique used to distinguish between
positive grams
(+ve) and
negative grams
(-ve)
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how does the
gram stain technique
work
the different staining properties are due to differences in the
chemical compositions
of the cell walls
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what does a
gram positive
bacterial cell
look like
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what does a
gram negative
bacterial
cell look like
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give one word to describe the
peptidoglycan
cell wall of a
gram positive
bacterial cell
thick
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give one word to describe the
peptidoglycan
cell wall of a
gram negative
bacterial cell
thin
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which bacterial cell has
lipopolysaccharides
,
gram positive
or
gram negative
.
gram negative
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what can be found on both the positive and negative
gram
cell walls at the bottom
plasma membranes
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how many
layers
are found in a
gram positive
bacterial cell
2
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what 2 layers are found in a gram positive bacterial cell
a thick
peptidoglycan
layer and the
plasma membrane
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what does a
gram negative
bacterial cell have on the top layer that the
gram positive
doesn't
an outer
lipopolysaccharide
membrane
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what difference does a
negative gram
having a
outer lipopolysaccharide membrane
have on the outcome of the gram stain experiment
the outer lipopolysaccharide membrane will absorb the
counter stain
safranin and appear red
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what difference does a
negative gram
having a outer
lipopolysaccharide
membrane have on the properties of the bacterial cell
it will make then non susceptible to some
antibiotics
such as
penicillin
or
lysozyme
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what is found in the thick
peptidoglycan
layer of a
positive gram
bacterial cell
long acidic
polysaccharides
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what is found attached to the
lipopolysaccharide
membrane of a
negative gram
bacterial cell
polysaccharide chains attached to the
lipids
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why is
penicillin
relatively safe for humans to take
human cells
do not have cell walls and they do not make
petidoglycan
,therefore there is no effect on human cells
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