The sign stimulus in this case was the red underbelly of the male stickleback and if this was covered, they were not attacked. The fact that all male stickleback engaged in this behaviour suggests it is invariant and a strong argument for the behaviour being biologically determined (nature). This behaviour is also believed to be adaptive as it increases evolutionary fitness by warding off other males from their nest while remaining inviting for female stickleback fish who do not have a red underbelly.