Light microscopy

Cards (26)

  • What units should you be able to work with by the end of this video?
    Micrometers and nanometers
  • Why might working with micrometers and nanometers seem tricky?
    They are smaller than millimeters
  • How many millimeters are in one meter?
    1,000 millimeters
  • What is the approximate length of a millimeter in biological terms?
    It is a very large size
  • What is a micrometer?
    One thousandth of a millimeter
  • How long is a bacterium like E. coli?
    About one micrometer
  • What is smaller than a micrometer?
    A nanometer
  • What is the diameter of a typical ribosome?
    Around 25 nanometers
  • How do you convert millimeters to micrometers?
    Multiply by one thousand
  • How do you convert micrometers to nanometers?
    Multiply by one thousand
  • What type of microscope is commonly used in biology?
    Light microscope
  • When were the first light microscopes invented?
    In the 1600s
  • What are common features of both early and modern light microscopes?
    Eyepiece lens, stage, focusing dial
  • What is the magnification capability of early light microscopes?
    Around 300 times
  • What is the magnification capability of modern light microscopes?
    Around 1000 times
  • What is a significant advantage of light microscopes?
    They can be used on living cells
  • What is a limitation of using stains in light microscopy?
    They can kill the cells
  • What does resolution refer to in microscopy?
    The ability to distinguish two separate objects
  • How does low resolution differ from high resolution in microscopy?
    Low resolution shows blurred details, high shows clarity
  • What is the scientific definition of resolution in A-level biology?
    Minimum distance to see two objects separately
  • What is the limit of resolution for a light microscope?
    Around 200 nanometers
  • Why is the limit of resolution around 200 nanometers?
    Due to the nature of light
  • What is the wavelength range of visible light?
    Around 400 to 700 nanometers
  • What happens if two objects are closer than 200 nanometers?
    They cannot be seen as separate objects
  • What type of microscope can be used when light microscopes fail?
    Electron microscope
  • What are the uses and limitations of light microscopes?
    Uses:
    • Study living cells
    • Observe processes like cell division

    Limitations:
    • Resolution limited to 200 nanometers
    • Stains can kill cells