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Biology
Microscopes
Light microscopy
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Created by
kevin n
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Cards (26)
What units should you be able to work with by the end of this video?
Micrometers
and
nanometers
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Why might working with micrometers and nanometers seem tricky?
They are smaller than
millimeters
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How many millimeters are in one meter?
1,000
millimeters
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What is the approximate length of a millimeter in biological terms?
It is a very
large size
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What is a micrometer?
One thousandth of a
millimeter
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How long is a bacterium like E. coli?
About one
micrometer
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What is smaller than a micrometer?
A
nanometer
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What is the diameter of a typical ribosome?
Around 25 nanometers
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How do you convert millimeters to micrometers?
Multiply by
one thousand
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How do you convert micrometers to nanometers?
Multiply by
one thousand
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What type of microscope is commonly used in biology?
Light microscope
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When were the first light microscopes invented?
In the
1600s
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What are common features of both early and modern light microscopes?
Eyepiece lens
,
stage
, focusing
dial
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What is the magnification capability of early light microscopes?
Around
300
times
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What is the magnification capability of modern light microscopes?
Around
1000
times
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What is a significant advantage of light microscopes?
They can be used on
living cells
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What is a limitation of using stains in light microscopy?
They can kill the
cells
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What does resolution refer to in microscopy?
The ability to distinguish two separate
objects
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How does low resolution differ from high resolution in microscopy?
Low
resolution
shows
blurred
details, high shows
clarity
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What is the scientific definition of resolution in A-level biology?
Minimum distance
to see two objects separately
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What is the limit of resolution for a light microscope?
Around 200
nanometers
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Why is the limit of resolution around 200 nanometers?
Due to the nature of
light
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What is the wavelength range of visible light?
Around 400 to 700
nanometers
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What happens if two objects are closer than 200 nanometers?
They cannot be seen as
separate
objects
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What type of microscope can be used when light microscopes fail?
Electron microscope
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What are the uses and limitations of light microscopes?
Uses:
Study
living cells
Observe processes like
cell division
Limitations:
Resolution limited to
200 nanometers
Stains
can kill cells
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