prac 4 cell membrane permeability

Cards (5)

2 factors that affect membrane permeability
temperature
concentration of solvents
how is beetroot used to measure membrane permeability ?
higher permeability means more pigment leaks out
lower permeability means less pigment leaks out
colorimeters are used to measure concentration of pigment
what is the method
cut 6 beetroot cores
place into 6 test tubes of distilled water
place into 6 water baths of different temperature
leave for 20 mins
filter the solutions into cuvette tubes
measure light absorbance with a colourimeter
risks  
ethanol is an irritant and flammable - goggles and keep away from naked flames
scalpel - cut on flat surface
water baths
effect of temperature on membrane permeability
increasing temperatures increase membrane permeability