Capture a sample and count them, then mark each individual.
Step 2:
Release them back to their environment.
Step 3:
Return and recapture a second sample from the same population, count the number of individuals and the number of marked individuals recaptured.
Lincoln index:
n1 x n2 / nm.
n1 = number of individuals in first sample
n2 = number of individuals in second sample
nm = number of marked individuals in second sample.
Capturing the maximum number of individuals to maximise the chance of individuals that are recaptured.
A suitable time between capture and recapture so individuals can disperse back into the population and the population has not changed in number (death, reproduction).
The marks cannot interfere with the animals lives or cause them to be more likely to be predated and must remain visible.
Unpaired t tests are when means have been generated by two independent groups.
Paired t tests are when means have been generated by the same group.