Chromatography Paper & Thin layer

Cards (9)

  • Chromatography is a technique that uses a stationary phase and a mobile phase to separate a mixture
  • The mobile phase flows through the stationary phase carrying out the components in the mixture with it
  • How does chromatography works?
    It works as the mixtures are separated because each substance in the mixture will travel at different rate through the stationary base , depending on their relative attractions to the phase ( how soluble each mixture is)
  • Stationary phase in Chromatography is the..
    • Chromatography paper ( putting ink one the paper
  • Mobile phase is the…
    • Liquid solvent (As the water gets absorb by the paper the colours separate)
  • To get the Rf value =Distance travelled by substance
    ——————————————————-
    Distance travelled by solvent
  • To get the Rf value you get the distance travelled by the solvent and substance from the origin ( the pencil line)
  • Materials you need are ...
    • Beaker
    • Pin
    • Pencil
    • Chromatography paper
    • Ink spot
  • Thin layer chromatography
    • The stationary phase is a thin layer of un reactive substance supported on a flat
    • The Mobil phase is a solvent
    • Some advantages are : Mobil phase moves quicker and evenly though the stationary phase and chromatograms show greater separation of the components in the mixture