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Chemistry
Practicals
Chromatography
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Created by
Chloe Smith
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Cards (26)
What does chromatography literally mean?
Color
writing
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What is chromatography used for?
To
separate
materials in a
mixture
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How does chromatography work in different labs?
It works the same in
school
and
professional
labs
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What are the two phases in chromatography?
Mobile phase
and
stationary phase
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What determines how components in a mixture separate during chromatography?
Affinity for
stationary
or
mobile phase
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What is the stationary phase used in this chromatography experiment?
Paper
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How far from the base should the baseline be drawn?
About 2
centimeters
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Why do we use pencil to draw the baseline in chromatography?
Pencil colors do not dissolve in
mobile phase
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How many food colorings are used in this experiment?
Four
food colorings
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What are the known food coloring colors used in the experiment?
Blue
,
yellow
, and red
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What is the purpose of the capillary tube in the experiment?
To
suck
up
and
place
the
coloring
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How should the dots of food coloring be applied to the baseline?
Lightly and
allowed
to dry in between
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Why is it important to use a different capillary tube for each color?
To avoid
cross-contamination
of colors
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What is the mobile phase used in this chromatography experiment?
Water
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How does the mobile phase move in chromatography?
By
capillary action
up the paper
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What happens to the colors in the food dye during chromatography?
They either dissolve in
water
or stay on paper
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How long should the chromatography be left in the solvent?
About
15 minutes
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What does the pencil line near the top of the paper indicate?
The distance the
water
traveled
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How can you visually identify the components of the unknown food coloring?
By
comparing
it to
known
colors
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What does the RF value represent in chromatography?
Retention factor
of the colors
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How is the RF value calculated?
Distance
color traveled divided by solvent distance
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What is the distance the solvent traveled in the example?
101
millimeters
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What is the distance the yellow color traveled in the example?
20
millimeters
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What is the RF value for the yellow color?
20
101
≈
0.198
\frac{20}{101} \approx 0.198
101
20
≈
0.198
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What RF value was calculated for the blue color?
85
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What RF value was calculated for the purple color?
63
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