Enzyme experiment

• What is the first step in the enzyme experiment with substrate concentration?

  Set up 5 test tubes labelled 1 - 5
• What volumes of distilled water are used in the experiment?

  10, 7.5, 5 and 2.5 ml

  
• What is the purpose of liquid soap in the enzyme experiment?

  To facilitate the reaction
• Why are different pipettes used in the experiment?

  To avoid cross-contamination of solutions
• What diameter and length should the potato cylinders be?

  8 mm diameter and 40 mm long
• What should be done immediately after placing the potato cylinder in the test tube?

  Mark the height of the liquid and start the timer
• What is the significance of measuring the height of bubbles after 5 minutes?

  To assess the enzyme activity level
• What is the first step in the enzyme experiment with temperature?

  Set up 5 water baths at different temperatures
• What is the role of phenolphthalein in the temperature experiment?

  It indicates pH changes in the solution
• How much lipase is added to each test tube in the temperature experiment?

  2 cm3 of lipase
• What should be done after adding lipase to the test tubes?

  Place test tubes in corresponding temperature water bath
• What is the purpose of the water bath in the temperature experiment?

  To maintain a constant temperature
• Why is it important to let the boiling tube climatise?

  To ensure accurate temperature readings
• What is the final step in the temperature experiment?

  Repeat all steps with the other temperatures
• What is the first step in the enzyme experiment with pH?

  Label test tube with pH to be used
• What should be done after adding amylase to the test tube?

  Add 1 cm3 of buffer solution to the amylase
• Why is a control starch sample used in the pH experiment?

  To compare the reaction results
• How long should the test tubes be left in the water bath before mixing?

  5 minutes
• What is done after mixing the amylase and buffer solution with starch?

  Start the timer and add drops to the spotting tile
• What does it indicate if there is no color change after adding iodine?

  Starch has been completely broken down
• How many times should the pH experiment be repeated?

  Three times for a mean average
• What are the steps involved in the enzyme experiment with substrate concentration?

  1. Set up 5 test tubes labelled 1 - 5
  2. Measure different volumes of distilled water and H2O2
  3. Mix contents of each test tube
  4. Prepare potato cylinders
  5. Add liquid soap to each test tube
  6. Place potato cylinders in test tubes
  7. Mark liquid height and start timer
  8. Measure bubble height after 5 minutes
• What are the steps involved in the enzyme experiment with temperature?

  1. Set up 5 water baths at different temperatures
  2. Add lipase to test tubes and label
  3. Place test tubes in corresponding water baths
  4. Add phenolphthalein and lipid to boiling tubes
  5. Add buffer solution to boiling tubes
  6. Let boiling tubes climatise
  7. Measure and mix solutions
  8. Start timer and note time until pink color disappears
  9. Repeat for other temperatures
• What are the steps involved in the enzyme experiment with pH?

  1. Label test tubes with pH values
  2. Add amylase and buffer solution to one test tube
  3. Add starch to another test tube
  4. Place both in a 37°C water bath for 5 minutes
  5. Mix the solutions and start the timer
  6. Add drops to the spotting tile until no color change
  7. Repeat for other pH solutions