HUMAN HISTOLOGY INTRO

• Name the parts

  A) Ocular lens (Eye piece)

  B) Diopter adjustment

  C) Nose Piece

  D) Objective lens

  E) stage clip

  F) aperture

  G) Diaphragm

  H) condenser

  I) Illuminator (light source)

  J) head

  K) arm (carrying handle)

  L) Mechanical stage

  M) course adjustment

  N) fine adjustment

  O) stage controls

  P) base

  Q) brightness adjustment

  R) light switch

  
• Identify what type of light microscopy

  A) Bightfield

  
• Identify what type of light microscopy

  A) Phase Contrast

  
• Identify what type of light microscopy

  A) Darkfield

  
• Histology

  • The study of the tissues of the body and how these tissues are arranged to constitute organs.
  • Cells and Extracellular Matrix are two interacting components of a tissue.
  • EC forms complex structure, supports the cells and contains the fluid transporting in and out of the cell.
• Microscope

  • magnifies the image of such objects thus making them visible to the human eye.
  •  are used to observe the shape of bacteria, fungi, parasites and host cells in various stained and unstained preparations.
• Compound light microscope

  -is the most common microscope used in routine microscope examination
• Brightfield microscopy

  • Commonly used type of microscope.
  • The field of view is brightly lit so that organisms and other structures are visible against it because of their different densities
• Darkfield microscopy

  • field of view is dark and the organisms are illuminated.
  • A special condenser is used which causes light to reflect from the specimen at an angle.
  • used for observing bacteria such as treponemes (which cause syphilis and leptospira (which cause leptospirosis)
• Phase-contrast Microscopy

  • allows the examination of live unstained organisms.
  • special condensers and objectives are used.
  • alter the phase relationships of the light passing through the object and that passing around it
• Magnification

  -the power of the microscope to enlarge the image of an object
• only use lens paper to clean objectives
• Scanner

  • red
  • 4
  • 40x
• Low Power objective (LPO)

  • yellow
  • 10
  • 100x
• High power objective (HPO)

  • blue
  • 40
  • 400x
• Oil immersion objective (OIO)

  • black
  • 100
  • 1000x
• • Scanner
  • Low power objective

  Course knob (2)
• • High power objective
  • Oil immersion objective

  Fine knob (2)
• Eyepiece / Ocular

  • The specimen is viewed through the eye-piece.
  • It has a lens which magnifies the image formed by the objective.(10х)
• diopter adjustment

  • change the focus on one eyepiece to compensate for the difference in vision between your two eyes.
• microscope tube

  • Attached on the top of the arm to support the eye-piece on its upper end.
• nosepiece

  • Attached under the arm of the microscope tube.
• objectives

  • Where the image of specimen passes fist.
  • 4x, 10x, 40x, 100x
  • OIO is used to provide best image at high magnification with the use of immersion oil.
• mechanical stage

  • Holds the slide and allows it to be moved to the left, right, forward and backward using the stage controlers
  • Fitted with vernier graduations.
• condenser

  • illuminates the specimen and controls the amount of light and contrast
• coarse adjustment knob

  • alters this distance rapidly and is used to bring the specimen into the field of view using an objective having low magnification power.
• fine adjustment knob

  • changes the distance very slowly and permits better viewing of the object.
  • The movement should be smooth.
• rheostat

  • a knob that is turned clockwise in order to increase the light intensity, or counter-clockwise to reduce the light.
• • Adjust nosepiece to lowest setting (Lowest = shortest objective)
• tissue

  Group of

  cells with

  interrelated

  functions
• Epithelial tissue

  • Provide protection, and functions for

  absorption and secretion

  • The cells in the epithelium are tightly packed, bleaving no room for blood vessels within the epithelial tissue.