B3 Required practical

• What is the first equipment needed for the experiment?

  Amylase solution
• What is used to test for the presence of starch?

  Iodine solution
• Why are pH buffers used in the experiment?

  To create different pH conditions
• What temperature should the water bath be set at?

  37°C
• What is the purpose of labeling the test tubes?

  To identify different pH levels
• How do you prepare a starch solution?

  Mix starch with distilled water
• What should be done immediately after adding amylase?

  Start the stopwatch
• What does a blue-black color indicate when iodine is added?

  Presence of starch
• What does it mean when iodine stays yellow?

  No starch is left
• What is the independent variable in the experiment?

  pH of the buffer solution
• What is the dependent variable in the experiment?

  Time taken for starch to be broken down
• Why is it important to keep control variables constant?

  To ensure a fair test
• What safety equipment should be worn during the experiment?

  Safety goggles
• What should be done with chemicals after the experiment?

  Dispose according to teacher's instructions
• What is expected at pH 7 during the experiment?

  Fastest rate of starch breakdown
• What conclusion can be drawn about amylase activity and pH?

  Amylase activity is affected by pH
• What are the key variables in the experiment?

  • Independent Variable: pH of buffer solution
  • Dependent Variable: Time taken for starch breakdown
  • Control Variables:
  • Temperature
  • Concentration of amylase solution
  • Volume of starch solution
  • Volume of iodine solution
• What are the steps of the method for the experiment?

  1. Label test tubes for pH levels
  2. Prepare starch and amylase solutions
  3. Set up pH buffers
  4. Place test tubes in water bath at 37°C
  5. Add amylase and start stopwatch
  6. Test for starch presence with iodine
  7. Record results and repeat for each pH level
  8. Analyze data by plotting a graph
• What are the expected results at different pH levels?

  • pH 7: Fastest starch breakdown
  • pH 4: Slower starch breakdown
  • pH 10: Slower starch breakdown
• What is the significance of the experiment?

  • Demonstrates how pH affects enzyme activity
  • Helps understand digestive enzymes in the body