Haemophilus Influenzae

• What does H flu stand for?

  Haemophilus influenzae
• What type of organism is H.influenzae?

  Gram-negative organism
• What color does H flu appear in a Gram stain?

  Red or pink
• What shapes can H flu exhibit?

  Coccoid and bacilli shapes
• What is the classification of H flu?

  Gram-negative coccobacillus
• What is one of the virulence factors of H flu?

  IgA protease
• On what type of agar does H flu grow?

  Chocolate agar
• What is chocolate agar made from?

  Heated blood agar with lysed red blood cells
• What factors are supplied by lysed red blood cells in chocolate agar?

  Factor 5 and Factor 10
• How can H flu grow on traditional blood agar?

  By being near hemolytic organisms like staph aureus
• What does the starfish on Pamela's desk represent?

  Factor 5
• What is another name for Factor 5?

  Nicotinamide adenine dinucleotide (NAD)
• What is another name for Factor 10?

  Hematin
• What does the sign saying "ten rules of the office" represent?

  Factor 10
• How is H flu classified based on capsule production?

  Typable and non-typable strains
• What are typable strains of H flu?

  Strains that produce a capsule
• Why are typable strains more invasive?

  They resist phagocytosis
• What are non-typable strains of H flu?

  Strains that do not produce a capsule
• What does the "please respect property" sign represent?

  Poly ribose ribitol phosphate (PRP)
• What does the jello surrounding the stapler symbolize?

  A capsule surrounding a bacterium
• What is the vaccine for H flu type B(typeable strains) known as?

  H flu type B (Hib) vaccine

  Effective for those strains with a polysaccharide capsule.
• What does the Hib vaccine contain?

  Type B capsular polysaccharide conjugated to a protein
• What does the Hib vaccine induce?

  T cell dependent memory
• What diseases are caused by the type B strain of H flu?

  Epiglottitis, septic arthritis, meningitis
• What is the thumb sign associated with?

  Lateral neck x-ray in epiglottitis
• What does a cherry red epiglottis indicate?

  Inflammation in epiglottitis
• What does the character Creed represent?

  Hib causes meningitis
• What is the treatment for H flu infections?

  Ceftriaxone

  Rifampin prophylaxis for close contacts
• What prophylaxis should be given for close contacts of meningitis?

  Rifampin
• What do non-typable strains of H flu cause?

  Sinusitis, acute otitis media, conjunctivitis
• What does James's broken keyboard symbolize?

  Non-typable strains of H flu
• What does the smoke rising from James's keyboard represent?

  Aerosol transmission of H flu
• What does James's red eyes symbolize?

  Conjunctivitis caused by non-typable strains
• What are the treatment options for non-typable strains of H flu?

  Amoxicillin and clavulanic acid
• What virulence factor allows H flu to grow near Staphylococcus aureus?

  Poly ribose ribitol phosphate
• What is the significance of the character Duane in the office scene?

  He represents the importance of PRP
• What does the character Michael's laughter represent?

  Non-seriousness of H flu infections
• What does the character James's gaming represent?

  Non-typable strains causing acute otitis media
• What does the character Creed's sickle represent?

  Increased risk of Hib infections in asplenic patients
• What does the character James's headphones symbolize?

  Acute otitis media caused by non-typable strains