At night time, when photosynthesis can't take place, are the stomata normally open or closed? Why?
Closed
At night time there is no light for photosynthesis, so plants don't need to absorb carbon dioxide. This means they can close their stomata and conserve water.
If there is plenty of water in the leaf, guard cells become turgid, which opens the stomata. This allows carbon dioxide to enter the leaf, but also allows water to escape.
Which structures help to minimise water loss?
Guard cells which can close stomata
Waxy cuticle
The upper epidermis is transparent so that light can pass through it. This means that as much light as possible can reach the palisade mesophyll cells below, and be used in photosynthesis.