Microbiology

• Who is conducting the microbiology practical?

  1. Becks
• What type of bacterial culture is being used in the practical?

  A safe strain of E. coli
• Why must you handle the bacterial culture as if it were dangerous?

  To maintain safety protocols
• What is the first step before handling the microbe?

  Set up equipment and wash hands
• Why should the agar plate lid not be opened unnecessarily?

  To prevent contamination
• Where should you open the lid of the agar plate?

  Near the Bunsen burner
• How should the agar plate be labeled?

  At the bottom, not the lid
• What should be written on the agar plate besides the name of the bacteria?

  Today's date and initials
• Why is it important to store the agar plate lid up initially?

  To work on it without contamination
• What is the purpose of the Bunsen burner in the practical?

  To create a sterile area
• What must be done if hands touch non-sterile surfaces?

  Wash hands before continuing
• How should the pipette be handled to maintain sterility?

  By the blunt end only
• What should be done to the lid of the bottle before taking a sample?

  Flame the lid gently
• Why is it important to minimize the time the agar plate lid is open?

  To prevent contamination
• What is the purpose of spreading the bacteria over the agar plate?

  To ensure even growth
• What happens if the surface of the agar is broken?

  It will be obvious and affect growth
• What is the purpose of adding disinfectant to the spreader?

  To kill any remaining bacteria
• What indicates that the bacteria are growing on the agar plate?

  The plate becomes cloudy
• What is the purpose of the antibiotic disk in the experiment?

  To test its effectiveness against bacteria
• What does a clear ring around the antibiotic disk indicate?

  Bacteria were killed by the antibiotic
• How can the effectiveness of the treatments be compared?

  By measuring the diameter of clear areas
• What factors should be considered to ensure a fair test?

  Viscosity and diffusion of substances
• Why is it important to consider the application of results to the wider world?

  To understand treatment effectiveness for illnesses
• What should be done after the practical is completed?

  Disinfect the table
• What happens to the agar plate after a few days?

  It shows cloudy areas from bacterial growth
• Why do we store agar plates upside down?

  To prevent condensation on the agar
• What should be done if the disks fall off when turning the plate upside down?

  Ensure they are pressed down properly
• What temperature should the agar plates be incubated at?

  30 degrees Celsius
• What is the role of the aden teaching school alliance?

  To support teachers with practical science
• What is the significance of writing your name on the agar plate?

  To identify ownership of the sample
• How can results be shared among classmates?

  By repeating tests and comparing data
• Why is maintaining a sterile environment crucial in microbiology experiments?

  To prevent contamination of cultures
• What is the correct method for inoculating an agar plate?

  Spread bacteria evenly across the surface
• How do different substances affect bacterial growth?

  By inhibiting or killing bacteria
• What is the purpose of sealing agar plates with tape?

  To prevent contamination while allowing oxygen
• What is the significance of measuring clear zones around antibiotic disks?

  To evaluate the effectiveness of treatments